|
Reactivity/
Applications |
Human,Mouse,Rat/WB,IHC-p,ELISA |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Gene_Name/
Protein_Name |
GAPDH/Glyceraldehyde-3-phosphate dehydrogenase |
|
Human_gene_id/
Human_swiss_prot_no |
2597/P04406 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
1.00042e+008/P16858 |
|
Rat_gene_id/
Rat_swiss_prot_no |
24383/P04797 |
|
Immunogen |
Synthesized peptide derived from the N-terminal region of human GAPDH. |
|
Specificity |
GAPDH Polyclonal Antibody detects endogenous levels of GAPDH protein. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.04% sodium azide. |
|
|
Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH |
|
Predicted_band(Da)/
Observed_band(KD) |
/36 |
|
|
| Content |
|
Western Blot analysis of various cells using GAPDH Polyclonal Antibody diluted at 1：2000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
|
|
Western Blot analysis of varias cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody：Goat Anti-rabbit IgG HRP( diluted at 1:5000, 25°C, 1 hour) |
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