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Reactivity/
Applications |
Human,Mouse/WB,IHC-p,IF,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
WNT1/Proto-oncogene Wnt-1 |
|
Human_gene_id/
Human_swiss_prot_no |
7471/P04628 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
22408/P04426 |
|
Immunogen |
Synthesized peptide derived from Wnt-1 . at AA range: 270-350 |
|
Specificity |
Wnt-1 Polyclonal Antibody detects endogenous levels of Wnt-1 protein. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
WNT1; INT1; Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog |
|
Predicted_band(Da)/
Observed_band(KD) |
40982/45 |
|
|
| Content |
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Western Blot analysis of various cells using Wnt-1 Polyclonal Antibody diluted at 1：1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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Western Blot analysis of HT29 cells using Wnt-1 Polyclonal Antibody diluted at 1：1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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