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Reactivity/
Applications |
Human,Mouse,Rat/WB,IHC-p,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
UCHL1/Ubiquitin carboxyl-terminal hydrolase isozyme L1 |
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Human_gene_id/
Human_swiss_prot_no |
7345/P09936 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
22223/Q9R0P9 |
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Rat_gene_id/
Rat_swiss_prot_no |
29545/Q00981 |
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Immunogen |
Synthesized peptide derived from UCH-L1 . at AA range: 140-220 |
|
Specificity |
UCH-L1 Polyclonal Antibody detects endogenous levels of UCH-L1 protein. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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|
Storage_stability |
-20°C/1 year |
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Other_name |
UCHL1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1 |
|
Predicted_band(Da)/
Observed_band(KD) |
24824/28 |
|
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| Content |
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Western Blot analysis of various cells using UCH-L1 Polyclonal Antibody diluted at 1：2000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for anbitody retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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