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Reactivity/
Applications |
Human,Monkey/WB,IHC-p,IF,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
TOP2A/DNA topoisomerase 2-alpha |
|
Human_gene_id/
Human_swiss_prot_no |
7153/P11388 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
/Q01320 |
|
Immunogen |
Synthesized peptide derived from Topo IIα . at AA range: 1-80 |
|
Specificity |
Topo IIα Polyclonal Antibody detects endogenous levels of Topo IIα protein. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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|
Storage_stability |
-20°C/1 year |
|
Other_name |
TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme |
|
Predicted_band(Da)/
Observed_band(KD) |
174385/174 |
|
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| Content |
|
Western Blot analysis of various cells using Topo IIα Polyclonal Antibody diluted at 1：1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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Western Blot analysis of 293 cells using Topo IIα Polyclonal Antibody diluted at 1：1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for anbitody retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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